Fig. 5
NF11217 and NF10547 contain novel Tnt1 insertions in NODULE INCEPTION (NIN) and PHOSPHOLIPASE C (PLC)-like, respectively. a A hybrid read example obtained for NF11217 with the causative Tnt1 insertion showing the Tnt1 border sequence (yellow) and PLC-like sequence (blue). b Genomic structure of PLC-like indicating the Tnt1 insertion site in NF11217. c Representative co-segregation data for Tnt1 insertions in NF11217 co-segregating with Nod + Fix- plants. The top panel shows PCR products from PCR reactions with two primers specific for PLC-like genomic sequences, Medtr4g085800-1 F and Medtr4g085800-1R, of 585 bp; the lower panel shows PCR products with one PLC-like genomic primer, Medtr4g085800-1 F, and one Tnt1 primer, Tnt-F1, of 805 bp. Lane 1 shows DNA markers (New England Biolabs, Beverly, MA; #N3232); lane 2, WT R108 DNA; and lanes 3-14, Nod + Fix- plants in the NF11217 BC1F2 population. d A hybrid read example obtained for NF10547 with the causative Tnt1 insertion showing the Tnt1 border sequence (yellow) and NIN sequence (blue). e Genomic structure of NIN indicating the Tnt1 insertion site in NF10547. f Representative co-segregation data for Tnt1 insertions in NF10547 co-segregating with Nod + Fix- plants. The top panel shows PCR products from PCR reactions with two primers specific for NIN genomic sequences, Medtr5g099060-1 F and Medtr5g099060-1R, of 503 bp; the lower panel shows PCR products with one NIN genomic primer, Medtr5g099060-1 F, and one Tnt1 primer, Tnt-R1, of 810 bp. Lane 1 shows DNA markers; lane 2, WT R108 DNA; and lanes 3–14, Nod + Fix- plants in the NF10547 BC1F2 population. Additional PCR genotyping data for NF11217 and NF10547 WT-like and Nod + Fix- plants from BC1F2 can be found in Additional file 12: Table S9 and Additional file 13: Table S10, respectively. Genotyping primers are listed in Additional file 15: Table S11