Rapid identification of causative insertions underlying Medicago truncatula Tnt1 mutants defective in symbiotic nitrogen fixation from a forward genetic screen by whole genome sequencing

Table 1 Segregation analyses of NF11217 and NF10547

Cross (Female × Male)	Generation	Total Plants	Phenotype		χ2 ^a
Cross (Female × Male)	Generation	Total Plants	Wild-type (Pink nodule and green shoot)	Nod + Fix- mutant (White nodule and purple plant)	χ2 ^a
NF11217 × R108	F₁	8	8	0	-
NF11217 × R108	F₂	328	258	70	2.756
NF10547 × R108	F₁	9	9	0	-
NF10547 × R108	F₂	335	248	87	0.168

^* χ ² analysis was calculated based on the expected 3:1 (wild-type: mutant) ratio, P > 0.05

ISSN: 1471-2164