Fig. 4

Validation of distinct embryonic myofiber type development between WZS and LR. a qPCR of MYHCI and MYHCIIA in WZS and LR at 4 stages.* p < 0.05, ** p < 0.005, ***p < 0.001 (LR vesus WZS, n = 3). b WB of LR and WZS at 28, 35, 42 dpc. GAPDH was used as control. Type II and typeI MYHC protein was detected by anti-myosin (skeletal, fast or slow) antibodies