Fig. 8

Flow cytometry analysis of immune cells in blood and lungs. Blood samples and lungs from infected C57BL/6J (grey bars), CAST/EiJ (green bars) and 129S1/SvImJ (pink bars) mice (1 × 10¹ FFU) were prepared. Cell suspensions were analyzed by flow cytometry on days 0, 3 and 5 p.i.. After excluding dead cells fluorochrome-labeled antibodies were used to differentiate various immune cell populations in blood (a) and lungs (b): CD4^pos (T helper cells); CD8^pos (cytotoxic cells); CD19^pos (B cells), NKp46^pos (NK cells), CD11b ^pos Ly6G^pos (granulocytes), CD115^pos (monocytes) and F4/80^pos (macrophages). The number of individual cell populations over time (mean +/−SEM) was determined. For each measurement lungs and blood samples from individual animals were used. Data from two independent experiments were combined (n = 6 per time point). Data of different time points were analyzed for statistically significant differences using non-parametric Mann–Whitney-U-test (*: p-value ≤ 0.05; **: p-value ≤ 0.01)