Fig. 1

Regulation of microarray expression in identified single astrocytes in culture. Double immunofluorescence confocal images of the astrocyte marker GFAP (red) and three proteins differentially regulated by cAMP (green) in control and treated cells. Mki67 and Ssat were analyzed in permeabilized astrocytes and NR2C in non-permeabilized cells. Nuclear Mki67 immunfluorescence was abundant in control astrocytes, but almost absent in cAMP-treated cells. Diffuse cytosolic Ssat and extracellular clusters of NR2C were intensely labeled in treated cells. Yellow signal in the Ssat labeling indicates colocalization with GFAP cytoskeleton. Note the star-shaped morphology acquired by astrocytes exposed to permeable cAMP agonists. Scale bar: 10 μm