Fig. 2

Effects of RSE capture primer spacing on target enrichment. a Schematic representation of the distribution of captured genomic DNA copy number obtained around the primer hybridization site, indicated with a red triangle, as measured by qPCRs, placed at increasing distances from the primer hybridization site and shown with black inverted triangles. Gray bars indicate captured random DNA fragments. b qPCR results for RSE extracted material at seven non-contiguous genomic regions, plotted as the copy number ratio of targeted sites (indicated as diamonds) to a common non-targeted region (beta actin). The amount of targeted vs. off-target material decreases within about 10 kbp of the RSE extraction site