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Table 2 qPCR correlation to sequencing coverage

From: The next generation of target capture technologies - large DNA fragment enrichment and sequencing determines regional genomic variation of high complexity

 

qPCR Probe Position within MHC

Corr. Coef

Corr. Coef

30362055

31417450

31682240

32016911

32935499

1&2

2&3

(1) non-Amped

1565

930

2569

1227

1010

0.94

 

(2) WGA

4,201,954

3,312,705

12,750,000

5,974,923

2,337,060

0.97

(3) Coverage Depth

166

130

372

253

95

 
  1. (1) & (2) results are copies of target per ul of extracted material