Fig. 3

Genes that are normally upregulated by HSF-1 independently of HS. a The Venn diagram shows the overlap among genes that were found to be significantly downregulated (q-value < 0.05) for each of the indicated comparisons between samples. The light purple shaded area includes genes that are downregulated upon treatment with hsf-1 RNAi, and are likely normally induced by HSF-1 independently of heat shock, therefore are referred to as upregulated genes. The q-value is the FDR-adjusted p-value of the test statistic, as determined by the Benjamini-Hochberg correction for multiple testing. b Hierarchical clustering of the genes normally upregulated by HSF-1 independently of HS. The fold change of genes found in the light purple section of the Venn diagram in (a) were determined by RNA-seq to be downregulated in response to hsf-1 RNAi, and would thus normally be upregulated by HSF-1. Lane 1 corresponds to the fold change of these genes in the control vs. hsf-1(-);-HS samples, and as a comparison, lane 2 corresponds to each the fold change of the same genes found in lane 1 but in the control vs. hsf-1(-);+HS samples. The heat map was organized using Cluster 3 by k-means and Euclidean distance. c Top processes normally upregulated by HSF-1 independently of HS. The genes found in the light purple section of the Venn diagram were classified by Gene Ontology terms that were determined using DAVID