Fig. 2

Stage-specific deep sequencing in 2-h intervals throughout early Platynereis embryogenesis. RNA-sequencing was performed on seven stages ranging from the zygote (2 h) to the protrochophore (14 h). Each time point sampled includes biological replicates, and there are also technical replicates (blue brackets) for the 4, 6, 8, and 14 h samples. The depth of these libraries range from 35 to 60 million total reads, represented with the blue bars. Filtered reads (red bars) are the total reads after trimming the adaptor sequences and low quality sections of the reads. The filtered reads were used to assemble the transcriptome, and the green bars represent the number of reads mapped back to the assembly. The mapped fragments range from 79 to 91 % of the filtered reads