Fig. 5

Mean distance to Line of Decent (LOD). Maximum parsimonious phylogenetic trees for all clone types identified in the metagenomes as being the latest. The red line in the trees indicates the LOD wherefrom SNPs (numbers on branches) have been counted. The LOD is set from the root (created for P41M3 and P82M3 using PAO1 as out-group) to the divergence of latest sampled isolates. White circles indicate the earliest sampled isolates and black circles indicates the latest sampled isolates from each patient, the coloured circles are comparable to the colours of Fig. 1. For each patient a mean distance to LOD is indicated below the patient name to the right of the corresponding tree. The mean distance to LOD of P82M3 is significantly different from the other patients, p <0.01, Fisher’s Exact test with Holm correction