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Fig. 4 | BMC Genomics

Fig. 4

From: Transcriptomic changes in relation to early-life events in the gilthead sea bream (Sparus aurata)

Fig. 4

a Cluster analysis: Clustering of all (Phase 1 and Phase 2) sequences being either up- or down regulated during stress are shown. The optimal number of clusters was obtained by generating a scree plot with the numbers of clusters on the x-axes and the distance between groups on the y-axes. The plot suggests 3 clusters as the best solution (red circle). Transcripts were further classified by the K means method. The data points given were grouped in three main clusters. The average value of the cases contained in each cluster is shown by the group centroid. Discriminant analysis correctly classified 96.1 % of the original grouped cases. b Heatmap analysis of the three clusters separately comprising all transcripts (Phase 1 and Phase 2) either up- or down regulated. Rows represent the log2-fold expression changes of different transcripts and columns the experimental sampling points. The relative quantitative changes within the rows are represented in color: red indicates a relative higher expression whereas green indicates a relative lower expression level

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