Fig. 3

Gene expression as a function of chromosomal location. Map of chromosomal location (base pair identity) for the most highly expressed genes (top 10 % expression) in a) K. pastoris and b) K. phaffii. Black lines indicate gene expression level at 24 h time point during batch cultivation in methanol. Red lines indicate locations of GC-rich autonomously replicating sequence (GC-ARS) motifs identified by BLAST. c) Box and whisker plot of the relative distance to the nearest GC-ARS motif in K. pastoris and K. phaffii for genes expressed at average levels genome-wide (45–55 % of max expression) and for the most highly expressed genes (top 10 % expression). Histograms of relative distances to GC-ARS motifs are shown alongside box plots for each gene set analyzed