Fig. 3

Mutagenesis kinetics of CRISPR/Cas9-induced injected embryos from 2-cell stage until the sphere stage. a Melting curve analysis indicated that indels can be detected as early as 2 cell-stage and the proportion of embryos with indels within calpn1a coding sequence increases with time (a sample of 8 HRM curves are shown by timepoint). b Quantification of the percentage of embryos with indels within calpn1a coding sequence at different stages of development (n indicates the number of individual embryos sequenced per timepoint; at least two independent batches of embryos were used per timepoint). The blue line is an approximation of the trendline