Fig. 2From: The genome-scale DNA-binding profile of BarR, a β-alanine responsive transcription factor in the archaeon Sulfolobus acidocaldarius In vitro binding of BarR to ChIP-enriched regions. Electrophoretic mobility shift assays to test BarR binding to a set of fragments representing high-enrichment ChIP-seq targets. Targets are named according to the gene closest to or overlapping most with the ChIP-seq peak. BarR protein concentrations are indicated above each EMSA autoradiograph with: + = 0.25 μM; ++ = 1.25 μM and +++ = 2.5 μM. For each tested fragment, the left panel represents the experiment performed without addition of β-alanine, while the right panel represents the experiment performed in the presence of 1 mM β-alanine (indicated below)Back to article page