Fig. 3

Characterization of galanin mature peptide and GAL prohormone expression. a Alignment of vertebrate galanin peptides. Astatotilapia burtoni sequences have a bolded species label. Sequences supported by peptide sequencing are marked with asterisks (*). Alignment made with the ClustalW program. b The A. burtoni galanin peptide fragmented and sequenced via tandem MS. c–g Representative transverse sections showing GAL-expressing neurons in the brain and pituitary. GAL-expressing cells were found in the preoptic area c, nucleus of the lateral tuberalis (NLT) d, medial region of the nucleus of the lateral recess (NRL) and in the region dorsal to the nucleus of the posterior recess (NRP) e, region of the periventricular nucleus of the posterior tuberculum f, and in the hindbrain along the lateral border of the vagal lobe (VL) e. CC, cerebellar crest; ON, optic nerve; pit, pituitary. Scale bars = 100 μm c; 25 μm d–g