Fig. 10

qRT-PCR validation of the relative expression data of genes obtained in RNA-seq analysis. Expression levels of selected transcripts are shown in dark grey (RNA-seq) and light grey (qRT-PCR). Plain columns and dotted columns refer to the expression data in Selenio and Dorella, respectively. EF1α gene was used for transcript normalization of the signal intensity which is shown on the y-axis. The X-axis shows comparisons of the results of two analyses. Error bars show standard deviations for triplicate assays. Asterisks indicate that expression levels are significantly different between RNA-seq and qRT-PCR (unpaired t test, P < 0.05)