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Table 2 Applied homology search strategies for ncRNAs

From: Automated detection of ncRNAs in the draft genome sequence of a colonial tunicate: the carpet sea squirt Didemnum vexillum

 

Blast parameters

 

Strategy

-r

-q

-G

-e

-E

-W

TF

1

5

−4

10

NA

6

7

69 %

2

4

−5

3

NA

5

7

?

3

5

−4

25

NA

10

7

69 %

4

4

−5

12

NA

8

7

?

5(2)

4

−5

3

20

5

7

?

6(3)

5

−4

25

20

10

7

69 %

7(2)

4

−5

3

1000

5

7

?

8(3)

5

−4

25

1000

10

7

69 %

Default

1

-3

5

10

2

11

99 %

  1. -r: reward for a nucleotide match, -q: penalty for a nucleotide mismatch, -G: Cost to open a gap, -e: Expectation value, -E: Cost to extend a gap, -W: Word size. The Theoretical frequency (TF), is the expected frequency according to the established parameters [31]. The strategies 1, 2, 3 and 4 were suggested by [32], while the others were modifications of the strategies written parentheses, according to [31]. The ‘Default’ strategy represents the predetermined parameters for blastn program