Fig. 5From: The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dogCorrelation of qPCR analysis of tissue specific and/or enriched miRNAs. Tissue specific and/or enriched miRNAs were chosen according to tissues that lack or require additional biomarkers of organ status and were measured in 21 tissues from 3 female rats by qPCR. The qPCR data was compared to the sequencing raw counts for each miRNA. A perfect correlation is equal to − 1 because the Ct value decreases as sequencing abundance increases. Twenty seven of the thirty nine miRNAs tested correlated very well to the sequencing data with a correlation of − 0.8 to − 1.0, 8 miRNAs displayed a correlation of − 0.79 to − 0.57 and 4 miRNAs displayed poorer correlations from − 0.33 to − 0.22. MiRs-196c-5p and 206-5p were not amplified by qPCR and, therefore, did not display tissue specificity or enrichment by qPCRBack to article page