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Fig. 7 | BMC Genomics

Fig. 7

From: Transcriptome analysis reveals regional and temporal differences in mucosal immune system development in the small intestine of neonatal calves

Fig. 7

RT-qPCR validation of selected DE genes identified by RNA-Seq. The gene expressions detected by RT-qPCR are shown by line graphs (black: jejunum; grey: ileum) on the top and values are shown on the right Y-axis as relative expression (ΔCq). Lower ΔCq values represent higher gene expression levels and vice versa. The gene expressions detected by RNA-Seq are shown by bar graphs (black: jejunum; grey: ileum) on the bottom and values are shown on the left Y-axis as log2 (CPM). A, B, C - indicate significant expression difference detected via RT-qPCR between different ages in the jejunum. a, b, c - indicate significant expression difference detected via RT-qPCR between different ages in the ileum. E, F - indicate significant expression difference detected via RNA-Seq between different ages in the jejunum. e, f - indicate significant expression difference detected via RNA-Seq between different ages in the ileum. Data are presented as Mean ± Standard deviation. Significantly regional difference were not labelled in the figure. TLR2, TLR4, TLR6, TLR10 and IL10 were validated as IL-enriched genes by RT-qPCR (ANOVA, P < 0.05)

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