Fig. 2
From: A novel approach to assessing bisphenol-A hazards using an in vitro model system
Effect of BPA on MAPK (p38), PI3K (p85), and PKA signalings pathways in spermatozoa. (a) The bar represents the densities of phospho-MAPK (p38) in BPA-treated and control spermatozoa. (b) Representative western blot image of phospho-MAPK (p38) probed with specific antibody. (c) Densities of phospho-PI3K (p85) in BPA-treated and control spermatozoa. (d) Representative western blot image of phospho-PI3K (p85) probed with specific antibody. (e) Densities of PKA substrate species in BPA-treated and control spermatozoa. (f) Phospho-PKA substrates were probed with an anti-phospho-PKA antibody. Lane 1: control; lane 2: 0.0001 μM BPA; lane 3: 0.01 μM BPA; lane 4: 1 μM BPA; and lane 5: 100 μM BPA. Data are presented as mean ± SEM (3 replicates). Values with different superscript characters (A,B,a,b,α,β) indicate significant differences between the control and treatment groups as determined by one-way ANOVA (p < 0.05)