Fig. 2

Comparison of two de novo assemblers based on number and sizes of assembled contigs and phylogenetic tree of 21 ASGV isolates. Size distribution of identified viral contigs from ASGV-infected apple sample assembled by Trinity (a) and Velvet (b). Size distribution of identified viral contigs from pear sample assembled by Trinity (c) and Velvet (d). The green-, blue-, and red-colored bars indicate ASPV, AGCAV, and ASGV, respectively. The sizes of only the longest and the shortest contigs in each transcriptome are indicated. (e) The phylogenetic tree was constructed based on the genome sequences of 13 ASGV isolates and 8 CTLV isolates. We followed the original annotations for CTLV and PBNLSV, which were highly homologous to ASGV. The accession number and the name of each isolate were indicated. Detailed information for each isolate can be found in Additional file 10