Fig. 4
Metabolic stress affects the expression profile of intestinal stem cell genes. a Scatterplot analysis of the fold expression profiles of 151 intestinal stem cell (ISC) genes upon thapsigargin and nutrient starvation. b Overall fold induction of ISC genes compared to the total gene induction upon thapsigargin treatment and nutrient starvation. c List of ISC genes upregulated and downregulation more than 2 fold upon stress. d Cell proliferation was analyzed by flow cytometry by monitoring EdU incorporation during the last 2Â h of the 4Â h treatment. Representative profiles of thapsigargin-treated (blue), nutrient-starved (green) and untreated control organoids (black) are shown. e Quantification of cell proliferation is presented as mean fluorescence intensity (MFI)