Fig. 7

Splicing of retained introns in CLK4/1 upon ER stress. a Gene schematic showing splicing of detained introns (introns 3 and 4) upon control conditions vs. stress conditions (red dotted line). b Plots of expression values for CLK1/4 upon thapsigargin treatment and nutrient starvation. c Western blot analysis of CLK4 protein upon thapsigargin and KRB stimulation for 6 or 20 h, as compared to tubulin loading control (d) PSI values for splicing of introns 3 and 4 of CLK1/4. e Sashimi plots representing the splicing of introns 3 and 4 of CLK1/4. Values in red represented the amount of DI splicing, calculated by taking the value of exon 4 skipped / (average of intron 3 retained, intron 4 retained), taken as an average of three biological replicates, with the standard deviation values. A higher value corresponds to more splicing of introns 3 and 4 and consequently, more retention of exon 4