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Fig. 1 | BMC Genomics

Fig. 1

From: Transcriptome responses to temperature, water availability and photoperiod are conserved among mature trees of two divergent Douglas-fir provenances from a coastal and an interior habitat

Fig. 1

Analysis Pipeline. The sequence libraries were aligned (a) to a non-redundant set of two Douglas-fir PUT sets (b). After log-transforming the count data (c), linear random effect models were used to (d) detect sources of variation within the data. Differential expression between genotype and environment was investigated by linear mixed models (LMM) (e). Nested models were compared by an F-test with Kenward-Roger approximation [102]. Additionally the difference in AIC (dAIC) and R2 (dR2 ) was calculated. PUTs are only considered as responding to an environmental factor if the corresponding regressor increases the R2 by more than 0.2. Overrepresentation of GO categories within the differentially expressed PUTs was investigated (f)

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BMC Genomics

ISSN: 1471-2164

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