Fig. 7
From: Viral deep sequencing needs an adaptive approach: IRMA, the iterative refinement meta-assembler
The artificial mixture, variant calling, and phasing of variants for an influenza A(H3N2) M gene. Donor viruses (1) are mixed (2) in a 99:1 ratio with new variants called (3) for the mixed virus and pairwise tested for phasing and visualization (4) by heat map. Consensus and minority phases are colored red and blue corresponding to the consensus alleles of each parent donor virus. Single nucleotide variants are shown with a triangle and colored according to their phase. Independent phase SNVs—without linkage to other minority variants—have green and gray triangles