Small RNA and degradome profiling reveals miRNA regulation in the seed germination of ancient eudicot Nelumbo nucifera

Table 3 Differential expressed known miRNAs during the sacred lotus seed germination

Change	miRNA	0 h-std	12 h-std	24 h-std	36 h-std	72 h-std	Significance
Up-regulated	Nnu-miR156c-5p	6.68	10.04	10.59	12.26	8.62	*
	Nnu-miR157a	56.90	100.44	144.83	169.62	69.79	**
	Nnu-miR159a	164.04	434.63	1008.33	1065.40	334.58	**
	Nnu-miR160b	6.99	10.04	16.09	13.28	11.49	**
	Nnu-miR162a-3p	78.52	132.40	218.52	125.68	167.91	**
	Nnu-miR165a-5p	8.27	25.26	45.31	32.02	20.12	**
	Nnu-miR165a-3p	5100.3	10745.91	18344.31	10534.78	10529.28	**
	Nnu-miR166b-5p	0.64	1.52	6.78	6.47	6.57	**
	Nnu-miR166c	5106.39	10766.00	18370.99	10560.33	10551.04	**
	Nnu-miR166d	2117.83	5231.12	9055.48	5330.06	4818.39	**
	Nnu-miR167a-3p	0	0.3	1.27	1.70	0.41	*
	Nnu-miR2111b	2.86	8.83	8.89	6.47	7.39	**
	Nnu-miR319b	103.63	150.05	214.29	149.52	114.13	**
	Nnu-miR319d	736.57	1670.66	2534.59	1737.40	1320.68	**
Down-regulated	Nnu-miR1030	4.45	3.04	1.69	1.70	1.23	*
	Nnu-miR170a	33.06	15.52	29.22	29.63	12.73	**
	Nnu-miR171g	33.38	16.44	30.07	29.97	12.73	**
	Nnu-miR2111a-5p	137.01	98.01	99.10	122.62	55.83	**
	Nnu-miR393a-5p	57.86	39.87	42.77	37.13	28.74	**
	Nnu-miR393c	57.22	38.65	42.35	36.78	28.33	**
	Nnu-miR394a	61.67	19.18	28.37	30.99	22.58	**
	Nnu-miR394b	103.32	44.13	52.09	53.13	32.43	**
	Nnu-miR396b-3p	8.27	1.83	3.39	1.70	1.23	**
	Nnu-miR408a	13.35	6.09	8.05	12.26	5.34	**
	Nnu-miR408c	29.56	17.04	20.75	24.18	16.01	**
	Nnu-miR530a	4.45	3.04	1.69	1.70	1.23	*

"std" represents normalized expression level (Normalised expression, RPM) of miRNAs
"*" and "**" represnent significant differentially expression between 0 h and other hours, *p < 0.05, **p < 0.01

ISSN: 1471-2164