Fig. 1

Structure (a, b) and DAPC (c, d) analyses of 212 Cryptococcus gattii isolates identify the presence of identical VGIIa, VGIIb, and VGIIc genetic clusters. a In the Structure analysis, the ∆K statistic predicts that the optimal number of populations (K) is eight. b Structure-based individual assignment of the 212 C. gattii isolates into eight populations. The Y-axis represents individual membership coefficient, while the X-axis corresponds to the 212 individuals. Thirty-five VGIIa, 22 VGIIb, and 33 VGIIc isolates were identified. c DAPC analysis indicates that values of K between 4 and 16 represent an informative number of populations. K values below the Bayesian information criterion (BIC) cutoff value (dotted line) represent useful summaries of population structure. d When K = 8 individual population assignment into the VGIIa, VGIIb, and VGIIc populations was identical compared with Structure. e Stability and agreement of individual population assignment into the VGIIa, VGIIb, and VGIIc subpopulations for K between 5 and 9 between Structure and DAPC. VGIIa, VGIIb, and VGIIc individuals are colored coded as blue, orange, and grey, respectively, through different values of K