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Table 4 Non-digestive proteins identified by mass spectrometry

From: High throughput techniques to reveal the molecular physiology and evolution of digestion in spiders

Protein

Digestive Fluid (%)

Fasting (%)

Fed (%)

SPCS

Venom peptide isomerase (heavy chain)

0.53

N.I

N.I

None

 Venom allergen 5

0.44

N.I

N.I

Inc

 Venom allergen 5

0.22

N.I

N.I

Inc

 U24-ctenitoxin-Pn1a

1.82

1.6

0.38

17–18

 U24-ctenitoxin-Pn1a

0.11

N.I

N.I

22–23

 U24-ctenitoxin-Pn1a

0.09

0.06

0.35

17–18

 U24-ctenitoxin-Pn1a

0.08

0.2

0.02

19–20

 U24-ctenitoxin-Pn1a

0.04

0.08

Id

17–18

 U24-ctenitoxin-Pn1a

N.I

0.05

0.22

22–23

 U9-ctenitoxin-Pr1a

N.I

0.009

Id

Inc

Protease inhibitor U1-aranetoxin-Av1a

N.I

0.41

0.57

None

 U3-aranetoxin-Ce1a

N.I

0.06

I

None

 L-cystatin

1.2

0.26

N.I

17–18

 Cystatin A2

N.I

0.7

1.16

None

 Serpin B3

0.06

0.07

0.03

Inc

 Serpin B6

N.I

0.03

N.I

None

 Leukocyte elastase inhibitor

0.07

N.I

N.I

Inc

 Leukocyte elastase inhibitor

0.003

N.I

N.I

21–22

 Alpha-1 inhibitor 3

0.04

0.008

0.002

27–28

 Peritrophin-1

N.I

0.16

0.74

Inc

 Peritrophin-48

0.008

0.04

N.I

Inc

 Transferrin

0.2

0.09

0.63

Inc

 Soma ferritin

0.05

5.1

2.9

None

 Soma ferritin

N.I

0.21

0.08

Inc

sum of leucine-rich repeat-containing proteins

5.9

a

a

a

  1. The values are the means of the percentages from the normalized spectral abundance factor to each sample. The data from midgut diverticula are from three distinct biological replicates and only proteins identified in at least two samples were used for quantitative analysis. Digestive juice NSAF is the sum of all different periods of feeding (fasting, 1, 3, 9, 25, 30 and 48 h
  2. Abbreviations: SPCS signal peptide cleavage site, Inc incomplete N-terminal sequence, N.I protein not identified, Id identified in only one replicate
  3. aprotein not searched in that sample or SPCS not analyzed