Fig. 2
From: The green ash transcriptome and identification of genes responding to abiotic and biotic stresses
Gene discovery saturation curve. A step-up method of assembly was completed in order to determine if additional sequencing is likely to yield new transcripts. For increasing numbers of input reads, the total number of transcripts and proteins continued to increase (a). For the average length and N50 of the transcripts, additional reads induced small increases for transcripts while predicted ORFs were unchanging after 30 million input reads (b)