Skip to main content

Table 1 Strains used in RNA sequencing experiments and respective growth conditions

From: The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets

Condition name

Strain

Conditionsa

Concentration (mg L−1)

pH

MICb (μg mL−1)

Reference

CaCl2

MgCl2

FeCl2

Non-induced

Kp13

Original strain, no polymyxin B supplementation

25.0

12.5

0.0

7.0

32

[8]

PB

Kp13PolB

LB supplemented with 4 μg mL−1 of polymyxin B

25.0

12.5

0.0

7.0

64

This study

PB + high calcium

Kp13Ca

LB supplemented with 4 μg mL−1 of polymyxin B plus high [Ca2+]

75.0

12.5

0.0

7.0

64

This study

PB + no magnesium

Kp13Mg

LB supplemented with 4 μg mL−1 of polymyxin B without Mg2+

25.0

0.0

0.0

7.0

128

This study

PB + high iron

Kp13Fe

LB supplemented with 4 μg mL−1 of polymyxin B plus high [Fe2+]

25.0

12.5

75.0

7.0

64

This study

PB + low pH

Kp13pH

LB supplemented with 4 μg mL−1 of polymyxin B in acid pH

25.0

12.5

0.0

5.8

64

This study

  1. aThe concentration of polymyxin corresponded to the concentration used during the extraction of RNA for RNAseq. All experiments were performed in duplicate. b,Minimum inhibitory concentration of polymyxin B in the respective strain