Fig. 3

Junction versus average conservation. a) Upstream junction versus average conservation for all high-confidence (σ(Ψ)< 0.1) high Ψ (red) and low Ψ (blue) events (downstream results are similar). 99.8 % of all events fall into one of 3 regimes: high (> 0.5) junction and low average conservation (Old^-), high junction and average conservation (Old⁺), and low junction and average conservation (New). Small Gaussian noise was applied in the y direction to avoid superimposing all tissues for each exon. Pie charts of Ψ for each regime and the whole dataset, also including medium Ψ events (green), are superimposed. b) The same data broken down first by Ψ range and then by conservation regime