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Fig. 2 | BMC Genomics

Fig. 2

From: Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitors

Fig. 2

Gene expression during erythroid differentiation from adult and cord blood stem cells. a PCA of DE gene expression in cord blood- (CB-) derived differentiations in SEM-F. Genes were selected if they were DE between any two AB populations, or between any two CB populations (Additional file 5: Table S3A). The union of these two DE gene sets were then used to arrange the samples in the PCA, clustering the CB-erythroblasts together with the AB-erythroblasts shown in Fig. 1. CB-erythroblast populations were isolated with the same gating strategy used for AB-erythroblasts. The Euclidean distances relating to this PCA are available in Additional file 19: Table S7. b Hierarchical clustering analysis of the same AB or CB DE gene set, clustering by Euclidean distance and by gene. The colour bar on the left hand side denotes clusters of co-regulated genes. c The number of DE transcripts between AB-erythroblasts and the same CB-erythroblast population is shown to examine the fold change at each point during erythropoiesis. Blue bars depict genes that are expressed more abundantly in CB-erythroblasts, and red, in AB-erythroblasts. d Mean expression values of selected key erythroid genes during erythropoiesis in SEM-F, +/- standard error of the mean. ACTB and PAFAH1B2 were used to normalise the data since they were consistently expressed throughout erythropoiesis in this data (Additional file 4: Tables S2)

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