Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitors

Table 1 Standard Erythroid Media (SEM) components. Erythroid progenitors were cultured in two Standard Erythroid Medium modifications SEM-F or SEM-i as indicated in methods

Component added to IMDM (Biochrom)	Source	SEM-F			SEM-i
		Phase I (Days 1-8)	Phase II (Days 8-11)	Phase III (Day 11 onward)	Phase I (Days 1-2)	Phase II (Days 2-8)	Phase III (Days 8-14)	Phase IV (Day 14 onward)
Fetal Bovine Serum (FBS)	Sigma	2 %	2 %	2 %	3 %	3 %	3 %	3 %
Penicillin	Sigma	100 U/ml	100 U/ml	100 U/ml	100 U/ml	100 U/ml	100 U/ml	100 U/ml
Streptomycin	Sigma	100 μg/ml	100 μg/ml	100 μg/ml	100 μg/ml	100 μg/ml	100 μg/ml	100 μg/ml
Glutamine	Sigma	4E-3M	4E-3M	4E-3M	4E-3M	4E-3M	4E-3M	4E-3M
Human AB serum	Sigma	3 %	3 %	3 %	2 %	2 %	2 %	2 %
Folic Acid	Sigma	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml
Inositol	Sigma	40 μg/ml	40 μg/ml	40 μg/ml	40 μg/ml	40 μg/ml	40 μg/ml	40 μg/ml
Holotransferrin	Sigma	200 μg/ml	1 mg/ml	1 mg/ml	120 μg/ml	120 μg/ml	1 mg/ml	1 mg/ml
Human Recombinant Erythropoietin (Epo)	Janssen	3 U/ml	3 U/ml	3 U/ml	3 U/ml	3 U/ml	3 U/ml	3 U/ml
Insulin	Sigma	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml	10 μg/ml
Monothioglycerol	Sigma	1.6E-4M	1.6E-4M	1.6E-4M	1.6E-4M	1.6E-4M	1.6E-4M	1.6E-4M
Cholesterol-Rich Lipids	Sigma	40 μg/ml	40 μg/ml	40 μg/ml	40μg/ml	40μg/ml	40μg/ml	40 μg/ml
Heparin	Sigma	3 U/ml	3 U/ml	3 U/ml	3 U/ml	3U/ml	3U/ml	3 U/ml
Human Stem Cell Factor (SCF)	R&D Systems	100 ng/ml	100 ng/ml	0	100 ng/ml	100 ng/ml	100 ng/ml	0
Dexamethasone	Sigma	1E-6M	0	0	1E-6M	1E-6M	1E-6M	1E-6M
IL3	R&D Systems	1 ng/ml	0	0	5ng/ml	5ng/ml	0	0
FLT3 Ligand	R&D Systems	0	0	0	20 ng/ml	20 ng/ml	20 ng/ml	0
IL6	R&D Systems	0	0	0	10 ng/ml	0	0	0
Thrombopoietin	R&D Systems	0	0	0	20 ng/ml	0	0	0

IMDM Iscove’s Modified Dulbecco’s Medium; IL3 interleukin-3; BSA bovine serum albumin; FLT3 Fms-like tyrosine kinase 3; IL-6 interleukin-6

ISSN: 1471-2164