Fig. 1
miR-27b KD globally alters expression of the presynaptic transcriptome. a Ontology-based classification of 195 presynaptic genes targeted by nCounter probes. nCounter probes were also designed against a few validated miR-27b and Dicer1 targets. b miRZip-27b (but not a control shRNA) suppressed miR-27b, but not miR-181a in DIV14 mouse cortical neurons (n = 3, * p < 0.05, t-test). miRNA levels were measured by qPCR. c nCounter profiling of the presynaptic transcriptome in miR-27b KD and CT neurons (DIV14). Genes were ranked based on their differential expression. Only genes that were significantly up- or down-regulated (147 out of 205) are shown (n = 3, p < 0.05, t-test). The pie chart indicates the number of genes up- and down-regulated, unchanged, or with low counts (discarded). d,e Top 20 most down- and up-regulated genes. f miR-27b and miR-181a levels probed by qPCR in Dicer1 fl/fl cortical neurons (DIV14) transduced with a control (Cre-) or a Cre-expressing lentivirus (Cre+) (n = 3, * p < 0.05, t-test). g nCounter profiling of differentially-expressed genes in Cre+ vs Cre- Dicer1 fl/fl neurons. Only genes that were significantly up- or down-regulated are shown (n = 3, p < 0.05, t-test). Error bars indicate SD. See also Additional file 2: Table S2