Fig. 10From: De novo transcriptome sequencing of black pepper (Piper nigrum L.) and an analysis of genes involved in phenylpropanoid metabolism in response to Phytophthora capsici Histochemical analysis of lignin in stem cross-sections of control and P. capsici,-inoculated black pepper plants. Stem were stained with Wiesner reagents for detecting lignin. Pink staining with the Wiesner reagent indicates the presence of p-hydroxycinnamyl aldehyde end groups in lignin. a, b, e, f Sections from control and P. capsici –inoculated P. flaviflorum (resistant) stems. c, d, g, h Sections from control and P. capsici -inoculated P. nigrum (susceptible) stems. a, b, c, d Sections from control plants 10 d after treatment. e, f, g, h Sections from inoculated plants 10 d after treatment. Adjustments to magnification and illumination were made to allow optimal viewing of individual sections. a, c, e, g: 40×; b, d, f, h: 100×; Blue arrow showed xylem and numbers of lignified xylem bundles, vessels, and interfascicular fibres. Red arrow showed the screens and vessels in phloemBack to article page