Fig. 3

Estimation of fusion sensitivity as a function of distance of breakpoint from the 3′ end. a Diagram showing the strategy used to estimate the probability of detecting a fusion (i.e. sensitivity) at different distances from the 3′ end by enumerating the proportion of genes having a coverage ≥10x. A indicates that there are more than 10 reads at that particular position for that gene while a X indicates that there are not. b Sensitivity as a function of the distance from the 3′ end for the UHR sample at varying levels of degradation. Loess trend is shown for each sample and 95 % confidence intervals are shown in gray. c Estimated sensitivity (log scale) for five different fusions present in UHR that occur at different distances from the 3′ end at different RIN values. If the fusion was detected at the particular degradation level it is shown as a square and if it is not detected it is shown as a circle. Linear trend line is shown for each fusion and 95 % confidence intervals are shown in gray