Fig. 1
From: Co-activator candidate interactions for orphan nuclear receptor NR2E1
MARCoNI array validated with Atro box. Cell lysates from HEK293 cells expressing wild-type FLAG-NR2E1LBD or variant FLAG-NR2E1LBD (R274G or R276Q) protein truncations were prepared. Vehicle-treated cells were also included. These lysates were tested in the MARCoNI array, which contained a wild-type (PYADTPALRQLSEYARPHVAFS) and mutant (PYADTPAARQASEYARPHVAFS) Atro box as positive and negative controls, respectively. FLAG-NR2E1LBD interacted strongly with the wild-type Atro box and with significantly reduced affinity to the mutant Atro box. The FLAG-NR2E1LDB variants did not bind to either of the ATRO peptides. AU, arbitrary units; LBD, ligand binding domain; *, significantly different from wild-type (p < 0.01). n = 3