Fig. 2
From: Co-activator candidate interactions for orphan nuclear receptor NR2E1
Twenty six novel co-regulator interacting peptides identified as binding to NR2E1 LBD in the MARCoNI array. Cell lysates from HEK293 cells expressing wild-type FLAG-NR2E1LBD or variant FLAG-NR2E1LBD (R274G or R276Q) protein truncations were prepared. Vehicle-treated cells were also included. These lysates were tested in the MARCoNI array, which contained 154 peptides from 64 co-regulator proteins. Only the significant FLAG-NR2E1LBD binding interactions with 26 co-regulators (q < 0.05) are depicted in the histogram. The NR2E1LBD variants abolished the majority of binding interactions observed between wild-type NR2E1LBD and co-regulator peptides. However, triangles indicate peptides that did not interact significantly differently with NR2E1LBD wild-type and the variant(s) (q > 0.05). AU, arbitrary units; LBD, ligand binding domain; peptide ID: co-regulator UniProt ID_amino acid start_amino acid end