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Fig. 5 | BMC Genomics

Fig. 5

From: Co-activator candidate interactions for orphan nuclear receptor NR2E1

Fig. 5

NR2E1 interacted with P300 and androgen receptor. GST, or GST-NR2E1LBD were over-expressed in E.coli and incubated with HEK293 cell lysate containing different overexpressed proteins: (a) Myc-tagged P300 (P300-Myc), EGFP-tagged androgen receptor (AR-EGFP) and myc-tagged PRGC1 (Myc-PRGC1). After incubation with glutathione/sepharose beads, purified complexes were resolved by SDS-PAGE followed by western blot using anti-EGFP, and anti-myc antibodies. Note that P300 and AR do not bind to the GST control but are pulled-down with NR2E1-GST. Also note the absence of binding of PRGC1 to both GST control and GST-NR2E1. GST input represents images of Ponceau S-stained blots that were converted to black and white. b Immunoprecipitation was carried out using anti-P300, anti-EGPP, and anti-Myc antibodies. Complexes were resolved by SDS-PAGE followed by western blot. GST-NR2E1 was detected using anti-GST antibody. Note the binding of GST-NR2E1 to P300 and AR but not to PRGC1. GST, glutathione-S-transferase; IP, immunoprecipitation; WB, western blot

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