Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

Table 3 Sex-linked markers and sequences for eight presence-absence (PA) loci in R. clamitans

		Proportion with restrction fragment sequenced
Locus	Sequence	Tadpoles		Adults
Locus	Sequence	Females	Males	Females	Males
RaclCT014^ab	TGCAGTGTCTCTGAGGGTTTACTGGTGATCCAGCGCATG	0.27	0.58	0.00	0.92
RaclCT015^ab	TGCAGCATATGTGCGTACGGTCGGCGGGAAGGGGTTAAGCTATGTCCAGTGCCCTGCATG	0.00	0.58	0.09	0.94
RaclCT016^ab	TGCAGCTCAGTATCTCCGGCCTCTGTCTGTCCTGTCCTTGACAGCATG	0.00	0.62	0.04	0.92
RaclCT017^ab	TGCAGAAGTGCAGTGCATTGCTGTATGATTGGCCAAAGCATG	0.30	0.60	0.09	0.96
RaclCT018^c	TGCAGCAAGAGGTGAAAACAACCGCTGTTGGCAGCATG	0.00	1.00	0.04	0.89
RaclCT019^c	TGCAGTGCTTGAGATGGATCACACAGTGTGATCCATCTCAAAAACTGCGACTGTTGCATG	0.00	1.00	0.05	0.89
RaclCT020^c	TGCAGGCTGCAAAGAAGAAACGAGAAAGCTGCATG	0.00	1.00	0.13	0.94
RaclCT021^c	TGCAGCATTGCAGTGCATTGTTGTCTGATGATTGGGCAAGCATG	0.00	1.00	0.04	0.94

^aThese loci were originally identified as sex-linked with adult specimens but not tadpoles
^bThese markers are almost entirely unsequenced in male tadpoles from Forest 5. Restriction fragments for these loci were sequenced in all other male tadpoles
^cThese loci were originally identified as sex-linked with tadpole specimens but not adults. While perfectly sex-linked in tadpoles, they are less sex-linked in adults

ISSN: 1471-2164