Fig. 3From: Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocolsDensity plots comparing distribution of counts in the differentially expressed genes with the distribution of counts for all genes expressed in the controls. The distribution of average read counts (AveLogCPM) for each of the tested genes (as calculated in edgeR) is plotted (orange). a-d The distribution of AveLogCPM counts for the differentially expressed genes found in the SE vs PE comparison are superimposed (purple) for Experiments 1–4. The overlapping region is a combination of orange and purple. e-g The distribution of AveLogCPM counts for the differentially expressed genes found in the NS vs PE comparison are superimposed (green) for Experiments 2–4. The overlapping region is a combination of orange and green Back to article page