Fig. 2

WSD induces sustained alterations in skeletal muscle transcription. Soleus muscle biopsies were collected longitudinally, before and after exposure to the WSD and after CR, and then subjected to RNAseq gene expression analysis, as described in “Materials and Methods”. DEGs were identified using three independent comparisons: a–c WSD vs chow (WSD/CHOW); e CR vs WSD (CR/WSD); and a, b and d CR vs chow (CR/CHOW). a The number of upregulated (brown) and downregulated (blue) genes from WSD/CHOW and CR/CHOW categories in rhesus macaque genome. b Venn diagram shows an overlap between WSD/CHOW and CR/CHOW genes. Heat maps of top DEGs during the transition from chow to WSD (c), CR vs chow (d), and CR vs. WSD (e) Each column represents an individual animal