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Fig. 2 | BMC Genomics

Fig. 2

From: Impact of blood collection and processing on peripheral blood gene expression profiling in type 1 diabetes

Fig. 2

Gene expression in blood samples collected and processed using the PAXgene versus Tempus system. a Venn-diagram showing the number of genes that are expressed in the majority of samples (≥5 of 9 samples) collected and processed using either the PAXgene or Tempus system. Histograms show the Log2(normalized intensity) of genes in each group. Genes detected using both systems (middle panels) contain the most abundantly expressed genes, while genes that are detected using one system but not the other (left and right panels) are among the 10% of genes with the lowest expression levels. b Scatter plot showing significant correlation between the expression of genes measured in PAXgene-processed and Tempus-processed samples. Dots outside the black lines represent the 5% of genes (901 unique genes) that are upregulated (1.5%; 287 genes) or downregulated (3.5%; 614 genes) by ≥2-fold in samples processed using the PAXgene vs. Tempus system (P < 0.05; Moderated T-test with Benjamini-Hochberg correction). c Heatmap showing the hierarchical clustering of samples based on genes that are differentially expressed by at least 2-fold

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