Characterization of the peripheral blood transcriptome and adaptive evolution of the MHC I and TLR gene families in the wolf (Canis lupus)

Table 4 Positively selected sites across carnivorous TLRs as inferred by different methods

Locus	Size (aa)	-2lnΔL	PAML M8	FEL	REL	Total No. of sites	Sites located in LRRs	ω
TLR1	789	11.57**	629	69, 289, 291, 316, 629	289, 291, 316, 324, 326, 366, 395, 561, 629	10	8	0.527
TLR2	766	5.88	None	557, 609	487, 557, 609	3	1	0.348
TLR3	905	2.02	None	13, 270, 293	13, 69, 270, 279, 293, 344, 587	7	6	0.307
TLR4	833	7.24*	193, 295, 341	46, 193, 319, 562, 564	46, 58, 91, 139, 193, 295, 306, 308, 319, 321, 339, 341, 347, 364, 369, 384, 393, 394, 464, 505, 510, 514, 517, 562, 564, 620, 832	27	24	0.493
TLR5	858	1.60	None	63, 137, 609	None	3	2	0.252
TLR6	797	2.34	None	109, 631	None	2	1	0.491
TLR7	1050	N.A.	None	None	None	-	-	0.173
TLR8	1038	2.42	None	234, 457, 683, 689	202, 234, 390, 416, 457, 683, 689	7	7	0.226
TLR9	1032	10.70**	5	5, 457, 627	None	3	2	0.163
TLR10	807	N.A.	None	None	None	-	-	0.439

Positively selected codons inferred by more than one ML method are underlined. The overall ω for each TLR was calculated by the SLAC method. N.A. indicates not available because of a low -2lnΔL value. *p < 0.05, **p < 0.01

ISSN: 1471-2164