Fig. 3

Transcriptomic analysis of the HBV mouse model. The total RNA of liver tissue in the HBV (+) mouse and HBV (−) mouse were extracted at 1, 3 and 6-months post injection. Then, RNA-Seq analysis was performed to assess the differentially expressed genes. The experiments were performed in triplicate. a RPKM box plot shows the distribution of gene expression in different samples. The abscissa is the sample name, and the ordinate is log10 (RPKM). Each region of the box icon notes five statistics: the maximum value, Q3 quartile, the median, Q1 quartile, and the minimum value. b Venn diagram of up-regulated and down-regulated genes from the HBV (+) mouse liver tissues at 1, 3 and 6 months post-injection. c, e GO analysis of the differently expressed proteins. The up-regulated (c) and down-regulated (e) genes in the HBV (+) mouse were analyzed by Gene Ontology. The charts show an overview of the gene ontology analysis with up to 10 significantly enriched terms in the BP, CC, and MF categories. The cut-off of the P-value is set to 0.05. Terms of the same category are ordered by P-values. Left terms are more significant than the right terms. Information about the percentage and number of involved genes in a term is shown at the y-axis. The KEGG Pathway analysis of the differentially expressed proteins. The up-regulated (d) and down-regulated (f) genes in the HBV (+) mouse were analyzed by KEGG Pathway. The top 10 enriched processes are shown here. P value =0.01 and P value =0.05 as two selected cutoffs are highlighted in the figure