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Table 1 Knockout strains derived by pessimistic and optimistic models on the core E.coli metabolic network

From: Robust mutant strain design by pessimistic optimization

  K Knockouts Succinate production
P-ROOM 3 6pg →ru5p + co2 + nadph, oac + accoa →cit, ac →ac(ext) 76.97
  4 g6p →6pg + nadph, oac + accoa →cit, suc fum + fadh2, ac →ac(ext) 82.36
  5 g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh nadph 107.07
P-OptKnock 3 6pg →ru5p + co2 + nadph, oac + accoa → cit, ac →ac(ext) 76.97
  4 g6p →6pg + nadph, oac + accoa →cit, suc fum + fadh2, ac →ac(ext) 82.36
  5 g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh nadph 107.07
ROOM 3 dhap gap, g6p →6pg + nadph, fadh2 + 0.5o2 →2atp 93.92
  4 dhap gap, g6p →6pg + nadph, fadh2 + 0.5o2 →2atp, glyc(ext) → 108.22
  5 g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh nadph 115.58
OptKnock 3 g6p →6pg + nadph, mal →pyr + co2 + nadph, nadh nadph 110.179
  4 g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, nadh nadph 123.314
  5 g6p →6pg + nadph, 3pg + glu →ser + akg + nadh, nadh nadph, glyc glyc(ext), ac(ext) → 129.786