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Table 1 Overview of C. jejuni Tn5 mutant libraries

From: Essential genome of Campylobacter jejuni

Library

# Tn5 Mutants

Total Reads

Mapped Reads (%)

# Unique Insertions

Mean (± SE)

S-CJ11168

1,400,000

9,040,241

6,812,731 (75.36)

95,929

71.02 ± 0.90

S-CJ11168-D

1,400,000

6,920,934

6,448,244 (93.1)

79,178

81.44 ± 11.54

R-CJ11168-D

281,000

6,052,446

5,685,107 (93.93)

52,607

108.07 ± 13.89

R-CJ81176-D (a)

82,000

1,638,463

1,303,248 (79.54)

29,565

44.08 ± 5.89

*a- > CJ11168

  

1,493,694 (91.16)

32,623

45.79 ± 5.46

  1. Mean is the average reads per unique insertion in the Tn5 library with standard error (SE)
  2. S: Seed library, R: Reconstruction, D: DPO, CJ11168: C. jejuni NCTC 11168, CJ81116: C. jejuni 81–176
  3. *Tn5 library (R-CJ81176-D, a) when mapped against the donor strain C. jejuni NCTC 11168 genome (a- > CJ11168), a higher alignment rate was achieved (91.6%)
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BMC Genomics

ISSN: 1471-2164

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