Differential expression of genes in animal model | Biological impact noted in our animal model (Ref 13) |
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Increased expression of gene Sphk1 in the WT HO group compared to Sphk1 −/− HO and the RA controls. | Increased expression of mRNA and protein of SphK1 in the WT HO group compared to Sphk1 −/− HO and the RA controls. Severe lung injury leading to BPD lung morphology in WT HO. Lung injury protection in Sphk1 −/− HO. |
Increased expression of genesrelated to IL-6 such as Il6, Il6 receptor alpha and Il6 signal transducer in the WT HO group compared to Sphk1 −/− HO and the RA controls. | Increased levels of Il-6 measured in the BAL fluid of WT HO group compared to Sphk1 −/− HO and the RA controls. |
Increased expression of genes related to neutrophil activation such as L, E, P-Selectin and P-Selectin glycoprotein ligand-1 in the WT HO group compared to Sphk1 −/− HO and the RA controls. | Increased infiltration of neutrophils in the BAL fluid and lung tissue in the WT HO group compared to Sphk1 −/− HO and the RA controls. |
Increased expression of genes coding for proteins aggravating DNA damage and apoptosis such as Gadd and Diablo in the WT HO group compared to Sphk1 −/− HO and the RA controls. | Increased apoptosis in the lung tissue of the WT HO group compared to Sphk1 −/− HO and the RA controls. |
Increased expression of genes regulating inflammation in the WT HO compared to Sphk1 −/− HO and the RA controls. | Increased cell infiltration and concentration of protein in the BAL fluid of the WT HO group compared to Sphk1 −/− HO and the RA controls |