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Table 1 Validation of differential gene expression noted in the microarray by comparing with the biological impact noted in the same animal model

From: Expression profiling of genes regulated by sphingosine kinase1 signaling in a murine model of hyperoxia induced neonatal bronchopulmonary dysplasia

Differential expression of genes in animal model

Biological impact noted in our animal model (Ref 13)

Increased expression of gene Sphk1 in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased expression of mRNA and protein of SphK1 in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Severe lung injury leading to BPD lung morphology in WT HO. Lung injury protection in Sphk1 −/− HO.

Increased expression of genesrelated to IL-6 such as Il6, Il6 receptor alpha and Il6 signal transducer in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased levels of Il-6 measured in the BAL fluid of WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased expression of genes related to neutrophil activation such as L, E, P-Selectin and P-Selectin glycoprotein ligand-1 in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased infiltration of neutrophils in the BAL fluid and lung tissue in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased expression of genes coding for proteins aggravating DNA damage and apoptosis such as Gadd and Diablo in the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased apoptosis in the lung tissue of the WT HO group compared to Sphk1 −/− HO and the RA controls.

Increased expression of genes regulating inflammation in the WT HO compared to Sphk1 −/− HO and the RA controls.

Increased cell infiltration and concentration of protein in the BAL fluid of the WT HO group compared to Sphk1 −/− HO and the RA controls