Fig. 8

The transient expression of ScJAZ6 in Nicotiana benthamiana leaves. (a) Conductivity measurement of N. Benthamiana leaves infiltrated with 35S::ScJAZ6-containing Agrobacterium strain for 24 h. (b and c) The transcripts of ScJAZ6 and the immunity-associated marker genes in the N. benthamiana leaves at 24 h after infiltration. NtEF1-α was used for normalization of the transcript levels. NtHSR201, NtHSR203, and NtHSR515, hypersensitive response marker genes; NtNPR1, a salicylic acid pathway-related gene; NtPR2, NtPR-1a/c, and NtPR3, jasmonate pathway-associated genes; NtEFE26 and NtAccdeaminase, the ethylene synthesis-dependent genes. Mock, the Agrobacterium strain carrying 35S::00. All data points were expressed as the mean ± SE (n = 3). Different lowercase letters indicate a significant difference, as determined by the Duncan’s new multiple range test (p-value <0.05). (d) DAB (3,3′-diaminobenzidinesolution) staining of N. benthamiana leaves at 48 h after Agrobacterium strain infiltration. (e) The infection results of N. benthamiana leaves by Pseudomonas solanacearum and Fusarium solani var. coeruleum after infiltration with 35S::00 (control) or 35S::ScJAZ6-containing Agrobacterium strain. Disease symptoms of infected leaves were observed at 7 d post-inoculation