Table 2 Primers used in this study
primer | Forward primer (5′-3′) | Reverse primer (5′-3′) | Strategy |
|---|---|---|---|
ScJAZ1-Q | CCTGTTACTACCACTACTAC | ACAAGGCTTTAGATAGAGTT | qRT-PCR analysis |
ScJAZ2-Q | CTGAAGAAGGTGAACTCA | ATCTGACTACTAAGCAACA | qRT-PCR analysis |
ScJAZ3-Q | TTTAATTCGGTGGTTTCC | AATACATCTCTACAGTCTCT | qRT-PCR analysis |
ScJAZ4-Q | CTTCTACGGCGGCAAGAT | CTTGAGCGACACCTTCCT | qRT-PCR analysis |
ScJAZ5-Q | CGCTCTGATTCCTGTTCG | GTCTCTTCCTATAATCCTCTTCCT | qRT-PCR analysis |
ScJAZ6-Q | GCTGTTCCTCCCGTAAGT | GTTGTCACCCTTTCCTTTCTTT | qRT-PCR analysis |
ScJAZ7-Q | CGATTAGCAGTGATTTCAT | ACATCTCTACAGTCCTCT | qRT-PCR analysis |
ScJAZ6-cDNA | GGACGAGAAGGTGCTGA | GGCGCTAGGGCAAA | Gene cloning |
ScJAZ6-Subloc | TGCTCTAGAATGGAGAGGGACTTCCTG | CGGGATCCGATCTGTAGTTTCGTACT | Subcellular localization assay |
ScJAZ6-32a | CGGGATCCATGGAGAGGGACTTCC | CCCAAGCTTTCAGATCTGTAGTTTCGTACTG | Prokaryotic expression |
ScJAZ6–1301 | TGCTCTAGAATGGAGAGGGACTTCCTG | CGGGATCCTCAGATCTGTAGTTTCGTACT | Transient overexpression |
GAPDH | CACGGCCACTGGAAGCA | TCCTCAGGGTTCCTGATGCC | Reference genes |
NtHSR201 | CAGCAGTCCTTTGGCGTTGTC | GCTCAGTTTAGCCGCAGTTGTG | Transient overexpression |
NtHSR203 | TGGCTCAACGATTACGCA | GCACGAAACCTGGATGG | Transient overexpression |
NtHSR515 | TTGGGCAGAATAGATGGGTA | TTTGGTGAAAGTCTTGGCTC | Transient overexpression |
NtNPR1 | GGCGAGGAGTCCGTTCTTTAA | TCAACCAGGAATGCCACAGC | Transient overexpression |
NtPR-1a/c | AACCTTTGACCTGGGACGAC | GCACATCCAACACGAACCGA | Transient overexpression |
NtPR2 | TGATGCCCTTTTGGATTCTATG | AGTTCCTGCCCCGCTTT | Transient overexpression |
NtPR3 | CAGGAGGGTATTGCTTTGTTAGG | CGTGGGAAGATGGCTTGTTGTC | Transient overexpression |
NtEFE26 | CGGACGCTGGTGGCATAAT | CAACAAGAGCTGGTGCTGGATA | Transient overexpression |
NtAccdeaminase | TCTGAGGTTACTGATTTGGATTGG | TGGACATGGTGGATAGTTGCT | Transient overexpression |
NtEF1-α | TGCTGCTGTAACAAGATGGATGC | GAGATGGGGACAAAGGGGATT | Transient overexpression |